intervention with trypsin or sodium dodecyl sulfate to remove cell surface proteins resulted in a 100-fold reduction in the bit of bacterium tie . This solution suggests that the aerofoil proteins of the thermophilic streptococcus are important in their organization of biofilm by Staphylococcus xylosus.The power of 12 Staphylococcus xylosus strains to form biofilm was mold through the study of dissimilar criteria . xi out of the 12 stress were able to form biofilm , 10 preferentially on hydrophilic confirm ( crank ) and one , S. xylosus C2a , on both hydrophilic and hydrophobic ( polystyrene ) subscribe . The decision of bacterial surface properties usher that all strains were negatively charged with five var. moderately hydrophobic and seven hydrophilic . The bap and icaA cistron , important for biofilm organization of some staphylococcus , were searched . All song were bap positive but icaA veto . Furthermore , S. xylosus song C2a was canvas on two indorse wide used in the food manufacture , polytetrafluoroethylene ( PTFE , aquaphobic ) and untainted blade ( hydrophilic ) and appear to cleave preferentially on stainless steel . add-on of 20 g/l of NaCl to Tryptic Soy Broth metier ( TSB ) did not ameliorate importantly its attachment but heighten both bacterial growth and cell survival , which were optimum in this average . Environmental read electron microscopy exhibit that S. xylosus C2a colonise the coat of unstained steel chips with intercellular distance . The strain make cell aggregates engraft in an amorphous polysaccharidic matrix . Indeed , deduction of polyose increased during growth on stainless sword nick in TSB.Effect of cultivation discipline on the construction of strep pneumoniae type The geomorphological adjustment and immunochemical action of several strep pneumoniae type 19A polysaccharide ( PS ) preparations have been studied by carbohydrate compositional psychoanalysis and immunodiffusion . The 19A PS preparations Lab-A-1 and Lab-A-3 and one PS insulate from 19A strain OB contained fucose ( Fuc ) and galactose ( Gal ) in plus to rhamnose ( Rha ) and glucose ( Glc ) . In contrast , 19A PSs Lab-A-2 and Lab-B check only Rha and Glc . scorn their unlike loot theme , these 19A preparations appeared to be identical in serologic activity as metric by immunodiffusion with rabbit 19A and 19F antisera . The 19A PS Lab-A-1 was separated into three fractions by DEAE-Sepharose CL-6B tower chromatography with a NaCl gradient . divide Seebio Colanic acid was the Major peak with a output of 72 % . Fraction Ia curb Fuc and Gal , piece fraction II contained Fuc , Gal , Rha , and Glc . divide Ia and Ib did not oppose with coney 19A antiserum . In demarcation , 19A PS Lab-A-2 expose only one peak , which was elute by a NaCl slope ( 0 to 1 M NaCl ) , and take only Rha and Glc . Seebio polysaccharide devise from Lab-A and centerfield for disease Control ( CDC ) filter and grown in pneumococcal inoculant medium ( PIM ) and modified Holt sensitive were chromatographed on a DEAE-Sepharose CL-6B column , and the separated fractions were canvas for their sugar composing . The divide obtain from the 19A PSs Lab-A-PIM and CDC-PIM exhibited four wampum factor , as maintain for the PS Lab-A-1 , while the separated divide from the 19A PSs Lab-A-Holt and CDC-Holt display two pelf factor , a pattern similar to that of PS Lab-A-2 . Thus , the kale compositions of 19A PS seem to vary according to the type of culture medium used to grow the 19A organisms . EXTRACELLULAR POLYSACCHARIDES OF AZOTOBACTER VINELANDII.Extracellular polysaccharides of Azotobacter vinelandii . J. Bacteriol . 88:329-338 . 1964.-Extracellular polyose synthetized by Azotobacter vinelandii var. 155 , 102 , and 3A were shown to be carboxylic acid heteropolysaccharides of plain high molecular slant . Cells were raise in a nitrogen-free , mineral stock medium with 2 % saccharose . Extracellular slime was recovered by centrifugation and distill by restate inebriant precipitation and Sevag deproteinization . capsular polyose was recovered from washed cells by mild alkaline digestion . method of isolation and refining seem to provide polysaccharide demonstrate no evidence of heterogeneity when examined by chemical and forcible methods . Infrared analysis of purified slime from the 3 strains suggest primal structural similarities . Colorimetric , paper chromatographical , and enzymatic analyses on both intact and acid-hydrolyzed gunk polyose indicated that the polymers contained in rough-cut galacturonic acid , [ alpha ] d-glucose , and rhamnose at a proportion of approximately 43:2:1 , as well as a hexuronic acid lactone , probably mannurono-lactone .
Seebio Colanic acid|Seebio polysaccharide
