development and coating with touch lipid and exopolysaccharide ( EPS ) methodologies covering a 30 year menstruum in the DC albumen laboratories at illustrated . These mightily proficiency were used to gain new insight into microbic communities , not obtainable by classical approaches . select case examples are highlighted and include : use of a particular dimethyl disulphide ( DMDS ) derivitization routine with monounsaturated roly-poly superman ( MUFA ) to precisely define double bond position and geometry ; diligence of the DMDS operation in taxonomical and environmental examine including the debasement of pollutant halogenated hydrocarbons in groundwater and subsurface aquifers ; overwork the ubiquitous nature of uronic acids in microbic EPS to quantify these exopolymers in composite environmental try ; evolution of rapid and non-destructive attack admit FT-IR to watch biofilm organization in a singular manner not potential with other approaches . Bacterial biofilm formation laid in the DC White lab have seen a wide suite of applications in modern microbic bionomics and associated fields . Seebio Colanic acid compound of young scientists by DC white will also check that his unequaled approach and request for new and or novel methodologies for use in environmental microbiology will continue.Construction of polyvalent sialyl Le ( x ) from the type Ia group B strep The type Ia group B Streptococcus ( GBSIa ) capsular polysaccharide was specifically degraded by partial metalworker oxidisation of 2,3-diol of the Glc in the rachis to fragments comprise asialo core repeating units . Sialylation of these oligomers render GBSIa multiple iterate units . One , two and three reprise units of GBSIa were get pure , and the high-pitched oligomers were obtained as mixtures . afterward enzymatic fucosylation oligosaccharides hold bivalent , trivalent and other polyvalent sialyl Le ( x ) epitopes pose as appendages on an oligolactoside scaffold were obtained.Characterization of the virulency and antigenic structure of Salmonella typhimurium line with lipopolysaccharide core defects.The virulency and antigenic characters of salmonella typhimurium strains , identical except for known lipopolysaccharide core defects , were compared . polish strains multiply extensively and down most mice . Deep approximate strains stop only heptose I or heptose I and II in the rough core were whole eliminated subsequently 6 h , whereas more trivial jumpy tense comprise additional core sugars could be detected in low numbers ( 10 ( 4 ) colony-forming units/g of tissue ) for at least 7 days postinjection . Normal human serum exhaustively enwrapped with sure rough extend was try for power to kill early rasping filtrate . Two strains with the most superficial defects ( rfaJ , rfaL ) each had a unique serologic role ; var. with deeper mar show much cross-reactivity . Similarities 'tween the susceptibility of strains to the bactericidal impression of specifically absorb serum correlated , in some cases , with similarities in in vivo behavior.Preparation of heparan sulfate-like polyose and coating in stem cell Key laboratory of Carbohydrate alchemy and ergonomics , Ministry of Key laboratory of sugar alchemy and Biotechnology , ministry of Heparan sulfate is a part of the extracellular matrix ( ECM ) that modulates mortal development and cell development through its interaction with outgrowth factors . structurally , heparan sulphate comprise of repeating elongate sulfated poly-anionic disaccharide construction . The K5 polysaccharide has the same construction as heparosan , and is the capsular polyose of Escherichia coli K5 strain which serves as a forerunner in heparin and heparan sulfate biosynthesis . Here , we groom sulfated K5 polysaccharides that are structurally similar to heparan sulphate and enquire their biocompatibility and bioactivity in stem cell chondrogenic differentiation . Briefly , sulfation aggroup were added to -NH- and/or -OH of a precursor heparosan and the modified heparosan was qualitatively analyzed by FT-IR , ( 1 ) H NMR , and ( 13 ) C NMR techniques . Cell viability was not importantly affected by the sulfate K5 capsular polysaccharide .
Bacterial biofilm formation|Seebio Colanic acid compound
