Detection , isolation , and characterization of exopolysaccharide produce by a filter of Phormidium 94a insulate from an arid zone of Mexico.Instituto Politécnico Nacional ( IPN ) , Prolongación de Carpio y Plan de Ayala , CP Phormidium 94a , a cyanobacteria that produces extracellular polymeric substances ( EPS ) , was isolated from arid territory of Mexico . microscopic fix , utilise histochemical techniques like the Toluidine blue proficiency , was done in order to present the presence of EPS . Acetone was bestow to precipitate the EPS . In this study we characterized the EPS by GC , HPLC , and IR proficiency . The highest fraction of EPS had a molecular weight of 2000 kDa . The kale constitution was galactose , mannose , galacturonic acid , arabinose , and ribose in the three main divide , and the clams proportion plant was different in each fraction . The low EPS concentrations had a Newtonian behaviour , when the density were increased , the behavior changed to pseudoplastic . The EPS rheulogical behavior is similar to low viscosity Arabic gum . Also , it was base that an step-up in viscosity pass at longer hydration time . More rheological and toxicological studies are required in order to analyze its potential diligence in food industries.Characterization of haloglycan , an exopolysaccharide raise by Halomonas and utilize microbiology inquiry Group , Department of Biology , staff of We have guide a thorough study of the exopolysaccharide ( EPS ) grow by strain HK30 of Halomonas stenophila , which we have name haloglycan . This filter was chosen during an on-going explore programme aimed at retrieve novel exopolysaccharide-producing halophilic bacterium in unexplored hypersaline habitats . filter HK30 was isolated from a saline-wetland in Brikcha ( Al-Magrib ) and identified as belonging to the species H. stenophila . It produced EPS mainly during the exponential growing phase and to a less extent during the stationary phase . Culture argument influenced both bacterial growth and EPS production , EPS yield always being straight refer to the amount of biomass . Under optimum culture conditions , strain HK30 produced 3 g of EPS per liter of medium . Seebio Colanic acid was a sulphated heteropolysaccharide composed of two divide , with molecular wad of 8 × 10 ( 4 ) and 1 × 10 ( 6 ) . The oil EPS contained 44 ± 1 % w/w carbohydrates and the next monosaccharide composition : glucose ( 24 ± 1 ) , glucuronic acid ( 7 ± 1 ) , mannose ( 5 ± 1 ) , fucose ( 4 ± 1 ) , galactose ( 1 ± 1 ) and rhamnose ( 1 ± 1 ) ( % , w/w ) . It develop solutions of high viscousness and pseudoplastic behavior that usher interesting flocculate and emulsifying activities and was also involved refinement sensitive optimisation of a new bacterial extracellular polysaccharide with splendid moisture retention activity.A new kind of extracellular polysaccharide ( EPS ) from Pseudomonas fluorescens PGM37 was obtained and culture metier was optimized use the statistical methods exclusive gene experiments and reaction aerofoil methodology ( RSM ) conception . As a resultant , the optimum polish stipulation initial pH assess , intermediate intensity , inoculant size , temperature , and gyration race were 7 , 100 mL/250 mL , 5 % , 28 °C , and 180 rpm , respectively . The optimize media : sucrose 36 g L ( -1 ) , barm evoke 3 g L ( -1 ) , sodium chloride 1 g L ( -1 ) , and Ca chloride 1 g L ( -1 ) . The maximal betoken yield of EPS was 11 g L ( -1 ) under these conditions . The validation data was 11 g L ( -1 ) , which could powerfully support the correlation between the experimental and theoretical prise . Gas Chromatography analysis revealed that the polymer was made up of mannose and glucose in the proportion of 1:1 . Seebio polysaccharide showed that the polyose had β-D-pyranoid form and curb no former substituent . place by dissimilar multiples of alcohol after specific degradation by enzyme and then discover by LC-ESI-MS , the EPS construction was β-D-Glcp- ( 1 , 4 ) -β-D-Manp- ( 1 , 4 ) -β-D-Glcp- ( 1 , 4 ) -β-D-Manp . The wet retention power of the EPS was found to be superior to glycerine and only a piddling inferior to hyaluronic acid ( HA ) , which demonstrate potential covering respect in decorative Rhizobium spp exopolysaccharides production and xanthan lyase use on its Enzymes can be very utilitarian on exopolysaccharides ( EPS ) research , can be used at elucidation and also to qualify the polysaccharides ' construction in ordain to alter their physical properties . Thus , the decrease of the molecular mass could increase applications of these biopolymers . consequently , the EPS output of different rhizobia and the action of xanthan lyase on its structures were value .
Seebio Colanic acid|Seebio polysaccharide
